https://www.newyorker.com/magazine/2022/03/07/a-journey-to-the-center-of-our-cells
Posts Tagged ‘cryoEM’
A Journey to the Center of Our Cells | The New Yorker
April 9, 2022The secret lives of cells — as never seen before
January 25, 2022Avoiding the pitfalls of single particle cryo-electron microscopy: Einstein from noise | PNAS
September 28, 2019Avoiding the pitfalls of single-particle #cryoEM: Einstein from noise https://www.PNAS.org/content/110/45/18037 A great paper, explaining in simple terms how you can fool yourself with fancy computer algorithms. However, perhaps it’s a bit too honest – only a Nobelist could write this!
Cryo-EM structure of substrate-bound human telomerase holoenzyme | Nature
April 28, 2018#CryoEM structure of substrate-bound human telomerase holoenzyme https://www.Nature.com/articles/s41586-018-0062-x Quote: “2 flexibly RNA-tethered lobes: the catalytic core (w/ #TERT & conserved motifs of telomerase RNA) & an H/ACA RNP”
What to expect in 2018: science in the new year
January 13, 2018What to expect in ’18: science in the new year
https://www.Nature.com/articles/d41586-018-00009-5 Insights from cancer & ancient #genomes. Cures from #CRISPR. Progress in
#OpenAccess. Also, lots on outer space. But nothing on #cryoEM, #DeepLearning, #QuantumComputing or the brain connectome. HT @OBahcall
Cryo-Electron Tomography at Yale
December 23, 2017Cutting Edge: Building bridges between cellular and molecular structural biology
December 22, 2017Cutting Edge: Building bridges between cellular & mol. structural biology https://ELIFEsciences.org/articles/25835 quote: “The use of structured biological #annotation is not common practice in
#cryoEM…[but] by the end of the meeting there was a clearer appreciation of the importance of [this]”
QT:{{”
As previously explained, structured biological annotation is the association of data with identifiers and ontologies taken from well-established bioinformatics resources. The use of structured biological annotation is not common practice in the electron microscopy or structural biology communities. Therefore, ontology experts were invited to the workshop to explain why these are useful and what resources and tools are available for assigning annotations. Use-cases such as mouse imaging data helped to explain the principles and practice of structured biological annotation. By the end of the meeting there was a clearer appreciation of the importance of structured biological annotation for searching and linking imaging data across different scales, between different imaging and structural databases and with other bioinformatics resources.”
“}}
Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating – ScienceDirect
November 30, 2017QT:{{”
• Find 3.8 Å resolution cryo-EM structure of the ∼200 kDa magnesium channel CorA • Mg2+-free CorA exhibits dramatic loss of symmetry in the cytoplasmic domain …
• Inter-subunit Mg2+ is important for stabilizing the closed state of CorA “}}
#CryoEM Structures of the Mg++ Channel CorA Reveal Symmetry Break upon Gating https://www.ScienceDirect.com/science/article/pii/S0092867415017195 In closed conformation: 5 identical subunits arranged tightly around 5-fold axis & pore. In open: they lose bridging ions & open to differing degrees with helix-hinging motions.
Matthies D1, Dalmas O2, Borgnia MJ1, Dominik PK2, Merk A1, Rao P1, Reddy BG2, Islam S2, Bartesaghi A1, Perozo E3, Subramaniam S4. Cell. 2016 Feb 11;164(4):747-56. doi: 10.1016/j.cell.2015.12.055.
linkstream2 microblog 