The draft genome of sweet orange (Citrus sinensis) – Nat Genet.

January 24, 2014

The draft #genome of sweet orange: Nearly 30K genes in only ~370 Mb + #RNAseq to find key Vitamin C genes
http://www.nature.com/ng/journal/v45/n1/full/ng.2472.html

The authors present a draft genome of sweet orange (Citrus sinensis) which covers 87.3% of the relatively compact orange genome
(approximately 367 Mb). Self-alignment of the citrus genome sequences identified one ancient triplication event, which was shared with a number of diverse plants including Arabidopsis thaliana, and no recent whole genome duplication events partially explaining the compact size of its genome. A combination of short sequence repeat (SSR) and SNP markers revealed that sweet orange is an interspecific hybrid between pummelo and mandarin (1:3 in genome composition with female of pummelo origin). Characterization of the unique protein coding genes in the citrus genome and the transcriptome analysis (RNA-Seq and RNA-PET) derived from different tissues in the citrus plant were used to identify the specific genes that are involved in the accumulation of Vitamin C in its fruit (the rate limiting GalUR in the galacturonate pathway is present in 12 copies which are developmentally regulated). Overall, the genome has almost 30,000 genes.

The draft genome of sweet orange (Citrus sinensis).
Xu Q, Chen LL, …., Ruan Y.
Nat Genet. 2013 Jan;45(1):59-66.
PMID: 23179022

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