Posts Tagged ‘cryoEMsymp’

Quantifying the local resolution of cryo-EM density maps | Nature Methods

November 14, 2017

Quantifying the local resolution of #cryoEM density maps
https://www.Nature.com/articles/nmeth.2727 “Theory…based on the following idea: a L Angstrom feature exists at a pt…if a 3D local sinusoid of wavelength L is statistically detectable above noise at that point.”

QT:{{”
We propose a mathematical theory and an efficient algorithm for measuring local resolution that address all of the above limitations. The theory (Online Methods) is based on the following idea: a λ-Å feature exists at a point in the volume if a three-dimensional (3D) local sinusoid of wavelength λ is statistically detectable above noise at that point. A likelihood-ratio hypothesis test of the local sinusoid versus noise can detect this feature at a given P value (typically P = 0.05). We define the local resolution at a point as the smallest λ at which the local sinusoid is detectable, and we account for multiple testing with an FDR procedure.
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Welcome | ScienceHill-CryoEM

October 28, 2017

http://sciencehill-cryoem.yale.edu/
cryoem.yale.edu
https://medicine.yale.edu/ccmi/

Visualizing the molecular sociology at the HeLa cell nuclear periphery | Science

October 26, 2017

http://science.sciencemag.org/content/351/6276/969.long

#Visualizing the molecular sociology [of complexes] at the HeLa cell nuclear periphery, w/ #CryoET at 10 Ang. res. http://science.ScienceMag.org/content/351/6276/969.long

Science. 2016 Feb 26;351(6276):969-72. doi: 10.1126/science.aad8857.
Visualizing the molecular sociology at the HeLa cell nuclear periphery.
Mahamid J1, Pfeffer S2, Schaffer M2, Villa E3, Danev R2, Cuellar LK2, Förster F2, Hyman AA4, Plitzko JM2, Baumeister W1.