Bias from removing read duplication [eg from PCR amplification] in ultra-deep #sequencing
http://bioinformatics.oxfordjournals.org/content/early/2014/01/02/bioinformatics.btt771 pot. overcorrection issues
Zhou et al.
Bias from removing read duplication in ultra-deep sequencing experiments
Estimating variant allele frequency and copy number variations can be approached by counting reads. In practice, read counting is
complicated by bias from PCR amplification and from sampling coincidence. This paper assessed the overcorrection introduced while removing read duplicates. The overcorrection is a particular concern when the sequencing is ultra-deep and the insert size is short and non-variant.
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